Regensburg (Advanced, 2-day) * I am (your position) Undergraduate student PhD student PostDoc Facility Manager Reserach group leader (PD, Prof.) Technician * I am doing imaging experiments… make a selection…3x per week or more frequent2x per week1x per week3x per month2x per month1x per month7-11x per year4-6x per year1-3x per yearno imaging experience * So far, I was using some of the following programs to process/analyze my images I did not analyze images so far Fiji ImageJ Adobe Photoshop Gimp BioimageXD / ICY CellProfiler Matlab Imaris / Volocity other * I am doing research in the following areas: shortly describe if you are working with a specific imaging devices and which kind of samples (cells, animal, human patients,…) and shortly mention imaging related methods you are working with (e.g. confocal, brightfield, IF, ICC, IHC, tracking, co-localization,………) * I am interested in topics regarding the following specific image processing or analysis (multiple selection possible). Due to time reasons we need to restrict to the topics interesting for the majority of the participants. Basic 3D-reconstruction 3D measurements (volume, surface, distance, intensity) Automated counting and 2D measurements (area, length, shape,…) Correct intensity quantification of fluorescent images Densiometry (e.g. semi-quantitative analysis of Western blots) Analysis of histological sections, stainings (separating colors and measuring positively stained areas) and brightfield images First steps in ImageJ/Fiji macro recording for individual processing and analysis automation No specific analysis interest / want to get an overview * Select the one topic which you think is most important or interesting to you (majority decides on which one we will do during the workshop). Depending on the time we might also woks on more than only one topic. Statistical co-localization analysis Tracking of moving objects (particles, cells) Semi-automatic tracing of networks (neuronal networks, vessels,…) Insight into extended automation (manually customize your automation macros to some of your needs) I would prefer to work one afternoon on my own images under supervision (with suggestions, potential solutions)
