MPI Martinsried (3-day)

* I will use the following computer system during the course:

Windows (PC) MAC (OSX) Linux (might not be fully functional)

* I am doing imaging experiments…

* I consider my experience level with scientific images as…

very basic (need to learn about image handling and want to see basic analysis methods) basic (I worked with images but want to have info on image handling as well as different processing and analysis methods) intermediate (some info on basics are ok and then I’m interested in advanced processing and analysis methods) experienced (I know all about correct image handling, I am more interested in advanced processing and analsis methods)

* I am doing research in the following areas: shortly describe if you are working with a specific imaging devices and which kind of samples (cells, animal, human patients,…) and shortly mention imaging related methods you are working with (e.g. confocal, brightfield, IF, ICC, IHC, tracking, co-localization,………)

* For the remaining time we will talk about additional optional analysis methods. Please choose one of the following which seems most important/interesting for your work. Highest voting decides about the method we will include. Besides this we also might include time for individual solutions. Optional part 1

Basics in manual macro scripting for customized automation (manually adjust recorded macros) Statistical co-localization analysis (of objects from different fluorescent channels) I would prefer to work on my own images under supervision in the remaining time to practice the learned

* Optional part 2

Detailed automated tracking and analysis of moving objects in 4D 3D Network Tracing (analyzing neuronal networks or vessels in 3D space) 3D-Segmentation (extracting features/objects from 3D images, e.g. tumors in medical MRI-stacks) and 3D measurements (volume, surface, intensity, object distances) I would prefer to work on my own images under supervision in the remaining time to practice the learned Basics in manual macro scripting for customized automation (manually adjust recorded macros) Advanced Macro Programming (based on basics from option above)

* I have the following question or problen when analyzing or preparing my scientific images (best send an example by mail beforehand)

BioVoxxel

Scientific Image Processing and Analysis

MPI Martinsried (3-day)