2-day workshop Düsseldorf (MEDRSD) * I am (your position) Undergraduate student PhD student PostDoc Facility Manager Reserach group leader (PD, Prof.) Technician * During my work I use the following computer system (to provide you with software you can use afterwards): Windows (PC) MAC (OSX) Linux (might not be fully functional) * I am doing imaging experiments… make a selection…3x per week or more frequent2x per week1x per week3x per month2x per month1x per month7-11x per year4-6x per year1-3x per yearno imaging experience * So far, I was using some of the following programs to process/analyze my images I did not analyze images so far Fiji ImageJ Adobe Photoshop Gimp BioimageXD / ICY CellProfiler Matlab Imaris / Volocity other * I consider my experience level with scientific images as… very basic (need to learn about correct image hanling and want to see rather basic analysis methods) basic (I have worked with images but want to have info about basic image handling and an overview over different processing and analysis methods) intermediate (some info on basics are ok and then I’m interested in advanced processing and analysis methods) experienced (I know how to handle scientific images, I rather want to see advanced processing and analsis methods) * I am doing research in the following areas: shortly describe if you are working with a specific imaging devices and which kind of samples (cells, animal, human patients,…) and shortly mention imaging related methods you are working with (e.g. confocal, brightfield, IF, ICC, IHC, tracking, co-localization,………) * I am interested in topics regarding the following specific image processing or analysis (multiple selection possible). Basic adjustments (contrast, image rotation and size adjustments) Background correction methods (including unequal lighting correction) Automatic counting Automatic 2D measurements (area, length, shape,…) Correct intensity quantification of fluorescent images First steps in ImageJ/Fiji macro recording for individual processing and analysis automation Do’s and Dont’s of publication figure preparation No specific analysis interest / want to get an overview I have a specific processing/analysis question and will send an e-mail with an example image and a short explanation I have the following question or problen when analyzing or preparing my scientific images * Which of the following optional topics is most interesting/important for you? (choose one) Analysis of histological sections and stainings (separating colors and measuring positively stained areas) Densiometry (e.g. semi-quantitative analysis of Western blots)
