Experience

 

Relevant software knowledge:

  • ImageJ and Fiji:
    • Extensive image processing in diverse fields of life sciences
    • Diverse image data analysis techniques
    • ImageJ macro programming and process automation
    • ImageJ plugin programming (Java) see also: BioVoxxel Toolbox
  • CellProfiler Analysis Pipelines
  • Photoshop / Gimp2:
    • Macro Programming
  • Inkscape/Corel Draw:
    • Vector graphic design
    • Figure design
  • Leica and Zeiss confocal microscope software and diverse CCD camera software
  • Nikon NIS-Elements
  • Zeiss AxioVision and LSM 5 Live ZEN 2007
  • Blender 3D (3D Models and 4D Animations)

Relevant experience in microscopy:

  • Confocal Laser Scanning Microscopy
    • Confocal live cell imaging
    • 3D imaging of fish embryo
    • 4D time-lapse microscopy in living, transgenic zebrafish larvae
      • Cell-cluster tracking
    • Fluorescence recovery after photobleaching (FRAP)
    • Fluorescence loss in photobleaching (FLIP)
    • Bimolecular fluorescence complementation (BiFC)
  • Extensive fluorescence and light microscopy
    • Opto-kinetic-response (OKR) test with zebrafish larvae
      • including programming of the test software
  • Experience with the following microscopy systems:
    • Leica TCS-SP5/AOBS
    • Zeiss LSM 510 Meta
    • Zeiss LSM 5 Live
    • Nikon Eclipse 90i (including epifluorescence)
    • Zeiss Axioplan2 imaging
    • Zeiss Axiovert 25C
    • Nikon SMZ 1000 stereo microscope

Relevant practical lab experience:

  • Extensive cloning of fluorescent fusion protein plasmids
  • Immuncytochemistry (single- and multi-color immunofluorescence)
  • Cryos- and paraffin sections
  • Immunhistochemistry
  • Whole mount in situ hybridization
  • Whole mount immunostaining in zebrafish larvae
  • Westernblot and ECL imaging

Referee for:

  • European Spine Journal
  • European Cells and Materials
  • PloS ONE

Languages:

  • German
  • English
  • Spanish

Advanced Training – Fiji Programming Learnathon 2017 @ MPI-CPG Dresden: